Freeform Cell-Laden Cryobioprinting for Shelf-Ready Tissue Fabrication and Storage.

ABSTRACT: One significant drawback of existing bioprinted tissues is their lack of shelf-availability caused by complications in both fabrication and storage. Here, we report a cryobioprinting strategy for simultaneously fabricating and storing cell-laden volumetric tissue constructs through seamlessly combining extrusion bioprinting and cryopreservation. The cryobioprinting performance was investigated by designing, fabricating, and storing cell-laden constructs made of our optimized cryoprotective gelatin-based bioinks using a freezing plate with precisely controllable temperature. The in situ freezing process further promoted the printability of cell-laden hydrogel bioinks to achieve freeform structures otherwise inconvenient with direct extrusion bioprinting. The effects of bioink composi

SUBMITTER: Ravanbakhsh H 

PROVIDER: S-EPMC9173715 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature