Association between the single nucleotide variants of the mitochondrial cytochrome B gene (MT-CYB) and the male infertility.

ABSTRACT:

Background

Idiopathic male infertility can be attributed to genetic predispositions that affect sperm performance and function. Genetic alterations in the mitochondrial DNA (mtDNA) have been linked to certain types of male infertility and abnormal sperm function. Mutations in the mitochondrial cytochrome B (MT-CYB) gene might lead to some deficiencies in mitochondrial function. Thus, in the current study, we aimed to investigate the effect of mutations in the MT-CYB gene on sperm motility and male infertility.

Methods and results

Semen specimens were collected from 111 men where 67 men were subfertile and 44 were fertile. QIAamp DNA Mini Kit and REPLI-g Mitochondrial DNA Kit from QIAGEN were used to isolate and amplify the mitochondrial DNA. Followed by PCR and Sanger sequen

SUBMITTER: Saleh Jaweesh M 

PROVIDER: S-EPMC9174114 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature