Impairment of 7F2 osteoblast function by simulated partial gravity in a Random Positioning Machine.

ABSTRACT: The multifaceted adverse effects of reduced gravity pose a significant challenge to human spaceflight. Previous studies have shown that bone formation by osteoblasts decreases under microgravity conditions, both real and simulated. However, the effects of partial gravity on osteoblasts' function are less well understood. Utilizing the software-driven newer version of the Random Positioning Machine (RPM^SW), we simulated levels of partial gravity relevant to future manned space missions: Mars (0.38 G), Moon (0.16 G), and microgravity (Micro, ~10^-3 G). Short-term (6 days) culture yielded a dose-dependent reduction in proliferation and the enzymatic activity of alkaline phosphatase (ALP), while long-term studies (21 days) showed a distinct dose-dependent inhibition of min

SUBMITTER: Braveboy-Wagner J 

PROVIDER: S-EPMC9174291 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature