Cellular Heterogeneity and Cooperativity in Glioma Persister Cells Under Temozolomide Treatment.

ABSTRACT: We have observed a drug-tolerant/persister state in a human glioblastoma (GBM) cell line after exposure to temozolomide, the standard-of-care chemotherapeutic agent for GBM. We used a multicolor lentiviral genetic barcode labeling to follow cell population evolution during temozolomide treatment. We observed no change in the distribution of the different colored populations of cells in persister or resistant cells suggesting that pre-existing minor subpopulations, which would be expected to be restricted to a single color, were not amplified/selected during the response to the drug. We have previously identified four genes (CHI3L1, FAT2, KLK5, and HB-EGF) that were over-expressed during the persister stage. Single-cell analysis of these four genes indicated that

SUBMITTER: Rabe M 

PROVIDER: S-EPMC9174429 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature