Comprehensive Transcriptome Sequencing Analysis of Hirudinaria manillensis in Different Growth Periods.

ABSTRACT: Medical leeches are widely been used in biochemical and clinical medical studies, helping to restore blood circulation to grafted or severely injured tissue. Mostly, adult leeches are being used in the traditional pharmacopeia, but the gene expression profiling of leeches in different growth periods is not well-reported. So, in this study, we used transcriptome analysis to analyze the comparative gene expression patterns of Hirudinaria manillensis (H. manillensis) in different growth periods, including larval, young, and adult stages. We constructed 24 cDNA libraries from H. manillensis larval, young, and adult stages, and about 54,639,118 sequences were generated, 18,106 mRNA transcripts of which 958 novel mRNAs and 491 lncRNAs were also assembled as well. Furthermore

SUBMITTER: Shan H 

PROVIDER: S-EPMC9174698 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature