Induction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.

ABSTRACT: Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nuclear magnetic resonance and high-performance liquid chromatography techniques, respectively. From these analyses, it was found that, independently of the carbon source, L. mesenteroides NRRL B-512F produced dextransucrases of the same size and product specificity. The 5' ends of dextransucrase mRNAs isolated from cells grown under different culture conditions were identical. Based on this evidence, we conclude that dextransucrases obtained from cells grown on the various carbon

SUBMITTER: Quirasco M 

PROVIDER: S-EPMC91750 | biostudies-literature | 1999 Dec

REPOSITORIES: biostudies-literature