Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry.

ABSTRACT: To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.

SUBMITTER: Lewandowski TM 

PROVIDER: S-EPMC9175107 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature