Fluorine-induced polarity increases inhibitory activity of BPTI towards chymotrypsin.

ABSTRACT: Substituting the P₁ position in bovine pancreatic trypsin inhibitor (BPTI) is known to heavily influence its inhibitory activity towards serine proteases. Side-chain fluorinated aliphatic amino acids have been shown to alter numerous properties of peptides and proteins and thus are of interest in the context of BPTI. In our study, we systematically investigated the site-specific incorporation of non-canonical amino acids into BPTI by microwave-assisted solid-phase peptide synthesis (SPPS). Inhibitor activity of the variants was tested towards the serine protease α-chymotrypsin. We observed enhanced inhibition of two fluorinated BPTIs compared to wild type and hydrocarbon variants. To further investigate the complexes, we performed X-ray structure analysis. Our findings underline

SUBMITTER: Leppkes J 

PROVIDER: S-EPMC9175108 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature