A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatile in vivo and in vitro applications.

ABSTRACT: Acidic pH is critical to the function of the gastrointestinal system, bone-resorbing osteoclasts, and the endolysosomal compartment of nearly every cell in the body. Non-invasive, real-time fluorescence imaging of acidic microenvironments represents a powerful tool for understanding normal cellular biology, defining mechanisms of disease, and monitoring for therapeutic response. While commercially available pH-sensitive fluorescent probes exist, several limitations hinder their widespread use and potential for biologic application. To address this need, we developed a novel library of pH-sensitive probes based on the highly photostable and water-soluble fluorescent molecule, Rhodamine 6G. We demonstrate versatility in terms of both pH sensitivity (i.e., pK _a) and c

SUBMITTER: Swanson WB 

PROVIDER: S-EPMC9175114 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature