Expression of mammalian proteins for diagnostics and therapeutics: a review.

ABSTRACT:

Background

Antibodies have proven to be remarkably successful for biomedical applications. They play important roles in epidemiology and medicine from diagnostics of diseases to therapeutics, treating diseases from incessant chronic diseases such as rheumatology to pandemic outbreaks. With no end in sight for the demand for antibody products, optimizations and new techniques must be expanded to accommodate this.

Methods and results

This review discusses optimizations and techniques for antibody production through choice of discovery platforms, expression systems, cell culture mediums, and other strategies to increase expression yield. Each system has its own merits and demerits, and the strategy chosen is critical in addressing various biological aspects.

Conclusions

SUBMITTER: Mark JKK 

PROVIDER: S-EPMC9175168 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature