Blocking P2X purinoceptor 4 signalling alleviates cigarette smoke induced pulmonary inflammation.

ABSTRACT:

Background

Chronic obstructive pulmonary disease (COPD) is associated with elevated ATP levels in the extracellular space. Once released, ATP serves as danger signal modulating immune responses by activating purinergic receptors. Accordingly, purinergic signalling has been implicated in respiratory inflammation associated with cigarette smoke exposure. However, the role of P2X4-signalling has not been fully elucidated yet.

Methods

Here, we analysed the P2X4 mRNA expression in COPD patients as well as cigarette smoke-exposed mice. Furthermore, P2X4-signalling was blocked by either using a specific antagonist or genetic depletion of P2rx4 in mice applied to an acute and prolonged model of cigarette smoke exposure. Finally, we inhibited P2X4-signalling in macrophages derived fr

SUBMITTER: Schneider S 

PROVIDER: S-EPMC9175376 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature