Crystallization of ApoA1 and ApoE4 nanolipoprotein particles and initial XFEL-based structural studies.

ABSTRACT: Nanolipoprotein particles (NLPs), also called "nanodiscs", are discoidal particles with a patch of lipid bilayer corralled by apolipoproteins. NLPs have long been of interest due to both their utility as membrane-model systems into which membrane proteins can be inserted and solubilized and their physiological role in lipid and cholesterol transport via HDL and LDL maturation, which are important for human health. Serial femtosecond crystallography (SFX) at X-ray free electron lasers (XFELs) is a powerful approach for structural biology of membrane proteins, which are traditionally difficult to crystallize as large single crystals capable of producing high-quality diffraction suitable for structure determination. To facilitate understanding of the specific role of two apolipoprotein/lipid

SUBMITTER: Shelby ML 

PROVIDER: S-EPMC9175823 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature