Fidelity of peripheral blood for monitoring genomics and tumor immune-microenvironment in myelodysplastic syndromes.

ABSTRACT: The aim of this study is to investigate whether the peripheral blood (PB) can serve as a surrogate immune-microenvironment to bone marrow for genetic and immune monitoring in myelodysplastic syndrome (MDS). We compared the composition of T cell subsets and somatic mutation burden in 36 pairs of PB and matching bone marrow aspirate (BMA) using multi-parameter flow cytometry and NGS-based targeted sequencing analysis, respectively. Our immune-subset and NGS-based mutation analysis of BMA showed significant concordance with those of PB in MDS. Therefore, PB can provide easily accessible tumor immune-microenvironment for monitoring in the immune and genetic landscapes for MDS patients.

SUBMITTER: Lee SE 

PROVIDER: S-EPMC9175915 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature