Dataset Information

Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid.

ABSTRACT: The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnetic beads to specifically extract, preconcentrate and fluorescently detect IL-6 directly on-chip. We assess how the physical properties of the beads can be tuned to improve assay performance by enhancing mass transport, reduce non-specific binding and multiply the detection signal threefold by transitioning between packed and fluidization states. With the integration of a full ELISA protocol in a single microfluidic chamber, we show a twofold reduction in LOD compared to conventional methods along with a large dynamic range (10 pg/mL to 2 ng/mL). We additionally demonstrate its application to IL-6 detection in undiluted serum samples.

SUBMITTER: Alexandre L

PROVIDER: S-EPMC9176165 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid.

Alexandre Lucile L Bendali Amel A Pereiro Iago I Azimani Madad M Dumas Simon S Malaquin Laurent L Mai Thanh Duc TD Descroix Stéphanie S

Scientific reports 20220608 1

The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnetic beads to specifically extract, preconcentrate and fluorescently detect IL-6 directly on-chip. We assess how the physical properties of the beads can be tuned to improve assay performance by enhanci ...[more]

PMID: 35676309

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Project description:Interleukin-8 (IL8) is an important cytokine that plays a significant role in tumor growth and angiogenesis across various malignant tumors, including oral squamous cell carcinoma (OSCC). It is an important biomarker for oral cancer; therefore, its early and accurate detection in bodily fluid reduces morbidity and mortality rates in cancer patients. The work presents the development of a label-free microfluidic miniaturized electrochemical immunosensor for IL8 biomarker detection at low concentration in saliva samples. A rapid, sensitive and selective biosensing platform was developed for IL8 detection using a nano-ceria integrated microfluidic system. The synthesized nano-ceria particles (8.13 nm) were employed to enhance the electrochemical biosensing signal and sensitivity of the biosensor due to their high catalytic properties and large surface area. For this, microfluidic chip was prepared by Indium tin oxide (ITO) (3 × 4 cm) containing three electrode patterns of working, reference and counter electrode. These electrode patterns were developed using a maskless photolithography technique and a polydimethylsiloxane (PDMS) mold created a 200 μm wide microchannel which was bound to the susbtrate using plasma treatment. Spectroscopy and microscopy techniques were used to confirm the synthesis of nano-ceria. Furthermore, electrode surface modifications were achieved by immobilization of chemically activated antibodies of IL8, as verified by Fourier transform-infrared spectroscopy (FT-IR). Furthermore, differential pulse voltammetry (DPV) was utilized to investigate electrochemical parameters and conduct biosensing studies. The developed electrochemical microfluidic biosensing platform works for an IL8 antigen in the concentrations ranging from 0.004 to 10 ng mL-1 with a limit of detection (LOD) and limit of quantification (LOQ) of 0.0001 ng mL-1 and 0.0006 ng mL-1, respectively. Moreover, the developed electrochemical biosensing platform was validated using human saliva samples, achieving percentage recovery within an acceptable range.

Dataset Information

Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid.

Publications

Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid.

Similar Datasets

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