Cloning, purification, kinetic and anion inhibition studies of a recombinant β-carbonic anhydrase from the Atlantic salmon parasite platyhelminth Gyrodactylus salaris.

ABSTRACT: A β-class carbonic anhydrase (CA, EC 4.2.1.1) was cloned from the genome of the Monogenean platyhelminth Gyrodactylus salaris, a parasite of Atlantic salmon. The new enzyme, GsaCAβ has a significant catalytic activity for the physiological reaction, CO₂ + H₂O ⇋ HCO₃^- + H⁺ with a k_cat of 1.1 × 10⁵ s^-1 and a k_cat/K_m of 7.58 × 10⁶ M^-1 × s^-1. This activity was inhibited by acetazolamide (K_I of 0.46 µM), a sulphonamide in clinical use, as well as by selected inorganic anions and small molecules. Most tested anions inhibited GsaCAβ at millimolar concentrations, but sulfamide (K_I of 81 µM), N,N-diethyldithiocarbamate (K<

SUBMITTER: Aspatwar A 

PROVIDER: S-EPMC9176631 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature