Renal cancer stem cell-derived sEVs impair renal function by inducing renal cell ERS and apoptosis in mice.

ABSTRACT:

Background

Cancer stem cells (CSCs) have been confirmed to participate in tumorigenesis, development, and metastasis, and to affect the local environment in normal tissues. Extracellular vesicles derived from CSCs (CSC-EVs) affect the local environment, contributing to tumor metastasis. However, the effect of small extracellular vesicles (sEVs) from renal CSCs (RCSCs) on renal function has not been studied. This study aimed to establish the impact of RCSC-sEVs on the renal function.

Methods

RCSC-sEVs were isolated from cell lines and locally injected into C57 mouse kidneys to observe the effect of RCSC-sEVs on the renal function. 24-hour urinary protein and serum creatinine were examined for renal function evaluation. Periodic Acid-Schiff (PAS) and immunochemistry (IHC) stai

SUBMITTER: Wu R 

PROVIDER: S-EPMC9177253 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature