Constructing a heparin-modified penile decellularized scaffold to improve re-endothelialization in organizational reconstruction.

ABSTRACT:

Background

Due to the unique anatomy and complex function of the penis, the reconstruction of penile defect is fraught with great challenges. The current standard methods are limited by numerous complications and insufficient donor sites. While functional vascularized penile tissue engineering offers a novel way to address this problem, revascularization remains the primary concern.

Methods

In this study, a penile scaffold with associated modifications was constructed. The performance of decellularized penile scaffolds (DPSs) was improved by conjugation with heparin (HEP) and reseeding with human umbilical vein endothelial cells (HUVECs). There were three groups according to the modifications, including native DPSs, HEP-DPSs, HEP-HUVECs-DPSs. After perfusing with 1% Triton X

SUBMITTER: Zhang H 

PROVIDER: S-EPMC9177262 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature