High molecular weight DNA extraction strategies for long-read sequencing of complex metagenomes.

ABSTRACT: By offering extremely long contiguous characterization of individual DNA molecules, rapidly emerging long-read sequencing strategies offer comprehensive insights into the organization of genetic information in genomes and metagenomes. However, successful long-read sequencing experiments demand high concentrations of highly purified DNA of high molecular weight (HMW), which limits the utility of established DNA extraction kits designed for short-read sequencing. The challenges associated with input DNA quality intensify further when working with complex environmental samples of low microbial biomass, which requires new protocols that are tailored to study metagenomes with long-read sequencing. Here, we use human tongue scrapings to benchmark six HMW DNA extraction strategies that are based

SUBMITTER: Trigodet F 

PROVIDER: S-EPMC9177515 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature