Single-cell RNA sequencing of mast cells in eosinophilic esophagitis reveals heterogeneity, local proliferation, and activation that persists in remission.

ABSTRACT:

Background

Mast cells (MCs) are pleiotropic cells that accumulate in the esophagus of patients with eosinophilic esophagitis (EoE) and are thought to contribute to disease pathogenesis, yet their properties and functions in this organ are largely unknown.

Objectives

This study aimed to perform a comprehensive molecular and spatial characterization of esophageal MCs in EoE.

Methods

Esophageal biopsies obtained from patients with active EoE, patients with EoE in histologic remission, and individuals with histologically normal esophageal biopsies and no history of esophageal disease (ie, control individuals) were subject to single-cell RNA sequencing, flow cytometry, and immunofluorescence analyses.

Results

This study probed 39,562 single esophageal cells by singl

SUBMITTER: Ben-Baruch Morgenstern N 

PROVIDER: S-EPMC9177790 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature