GRHL2 motif is associated with intratumor heterogeneity of cis-regulatory elements in luminal breast cancer.

ABSTRACT: In breast cancer patients, tumor heterogeneity is associated with prognosis and therapeutic response; however, the epigenetic diversity that exists in primary tumors remains unknown. Using a single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq), we obtained the chromatin accessibility profiles of 12,452 cells from 16 breast cancer patients including 11 luminal, 1 luminal-HER2, 1 HER2⁺, and 3 triple-negative subtypes. Via this profiling process, tumors were classified into cancer cells and the tumor microenvironment, highlighting the heterogeneity of disease-related pathways including estrogen receptor (ER) signaling. Furthermore, the coexistence of cancer cell clusters with different ER binding motif enrichments was identified in a single ER⁺

SUBMITTER: Kumegawa K 

PROVIDER: S-EPMC9177858 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature