ZNF677 suppresses renal cell carcinoma progression through N6-methyladenosine and transcriptional repression of CDKN3.

ABSTRACT:

Background

Studies on biological functions of N6-methyladenosine (m⁶ A) modification in mRNA have sprung up in recent years. Previous studies have reported m⁶ A can determine mRNA fate and play a pivotal role in tumour development and progression. The zinc finger protein 677 (ZNF677) belongs to the zinc finger protein family and possesses transcription factor activity by binding sequence-specific DNA.

Methods

The expression of ZNF677 and its clinicopathological impact were evaluated in renal cell carcinoma (RCC) patients. The m⁶ A level of ZNF677 was determined by m⁶ A methylated RNA immunoprecipitation-sequencing (MeRIP-seq) and MeRIP-qPCR in RCC tissues and adjacent normal tissues. RNA immunoprecipitation-qPCR (RIP-qPCR) and luci

SUBMITTER: Li A 

PROVIDER: S-EPMC9178504 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature