Hypoxia promotes differentiation of pure cartilage from human induced pluripotent stem cells.

ABSTRACT: While cartilage can be produced from induced pluripotent stem cells (iPSCs), challenges such as long culture periods and compromised tissue purity continue to prevail. The present study aimed to determine whether cartilaginous tissue could be produced from iPSCs under hypoxia and, if so, to evaluate its effects on cellular metabolism and purity of the produced tissue. Human iPSCs (hiPSCs) were cultured for cartilage differentiation in monolayers under normoxia or hypoxia (5% O₂), and chondrocyte differentiation was evaluated using reverse transcription‑quantitative PCR and fluorescence‑activated cell sorting. Subsequently, cartilage differentiation of hiPSCs was conducted in 3D culture under normoxia or hypoxia (5% O₂), and the formed cartilage‑like tissues were evalu

SUBMITTER: Shimomura S 

PROVIDER: S-EPMC9178684 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature