Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H₂S in Holstein Cows with Clinical Mastitis.

ABSTRACT: H₂S plays an important role in various inflammatory diseases. However, the role of H₂S and synthetic enzymes in Holstein cows with CM is unknown. The aim of this study was to identify DEPs associated with sulfide metabolism and further investigate their roles in dairy cows with CM. From 3739 DEPs generated by data-independent acquisition proteomics, we identified a total of 17 DEPs included in 44 GO terms and five KEGG pathways related to sulfide metabolism, including CTH and cystathionine-β-synthase (CBS). Immunohistochemical and immunofluorescence staining results showed that CTH and CBS proteins were present mainly in the cytoplasm of mammary epithelial cells. Endogenous H₂S production in the serum of the CM group was significantly lower than that of the

SUBMITTER: Zhang B 

PROVIDER: S-EPMC9179249 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature