Effect of Different Basal Culture Media and Sera Type Combinations on Primary Broiler Chicken Muscle Satellite Cell Heterogeneity during Proliferation and Differentiation

ABSTRACT:

Simple Summary

Little consistency in the literature exists for optimal culture conditions for proliferating and differentiating primary broiler chicken muscle satellite cells regarding basal culture media, proliferation sera, and differentiation sera. This experiment assessed primary satellite cell proliferation and differentiation when cultured in different combinations of basal media and sera. Cells were cultured in different basal media: low glucose Dulbecco’s Modified Eagle’s medium, McCoy’s 5A, and high glucose Dulbecco’s Modified Eagle’s medium. Each media was supplemented with 15% chicken serum, or a combination of 5% horse serum + 10% chicken serum during proliferation while 3% horse serum or 3% chicken serum were supplemented during differentiation. Cultures were immunofl

SUBMITTER: Flees J 

PROVIDER: S-EPMC9179426 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature