Centrosome Positioning in Migrating Dictyostelium Cells.

ABSTRACT: Directional cell migration and the establishment of polarity play an important role in development, wound healing, and host cell defense. While actin polymerization provides the driving force at the cell front, the microtubule network assumes a regulatory function, in coordinating front protrusion and rear retraction. By using Dictyostelium discoideum cells as a model for amoeboid movement in different 2D and 3D environments, the position of the centrosome relative to the nucleus was analyzed using live-cell microscopy. Our results showed that the centrosome was preferentially located rearward of the nucleus under all conditions tested for directed migration, while the nucleus was oriented toward the expanding front. When cells are hindered from straight movement by obstacles, the c

SUBMITTER: Ishikawa-Ankerhold H 

PROVIDER: S-EPMC9179490 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature