The Actin Cytoskeleton Responds to Inflammatory Cues and Alters Macrophage Activation.

ABSTRACT: Much remains to be learned about the molecular mechanisms underlying a class of human disorders called actinopathies. These genetic disorders are characterized by loss-of-function mutations in actin-associated proteins that affect immune cells, leading to human immunopathology. However, much remains to be learned about how cytoskeletal dysregulation promotes immunological dysfunction. The current study reveals that the macrophage actin cytoskeleton responds to LPS/IFNγ stimulation in a biphasic manner that involves cellular contraction followed by cellular spreading. Myosin II inhibition by blebbistatin blocks the initial contraction phase and lowers iNOS protein levels and nitric oxide secretion. Conversely, conditional deletion of Arp2/3 complex in macrophages attenuates spreading and in

SUBMITTER: Ronzier E 

PROVIDER: S-EPMC9180445 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature