Blood Clots versus PRF: Activating TGF-β Signaling and Inhibiting Inflammation In Vitro.

ABSTRACT: The preparation of platelet-rich fibrin (PRF) requires blood centrifugation to separate the yellow plasma from the red erythrocyte fraction. PRF membranes prepared from coagulated yellow plasma are then transferred to the defect sites to support tissue regeneration. During natural wound healing, however, it is the unfractionated blood clot (UBC) that fills the defect site. It is unclear whether centrifugation is necessary to prepare a blood-derived matrix that supports tissue regeneration. The aim of the present study was to compare lysates prepared from PRF and UBC based on bioassays and degradation of the respective membranes. We report here that lysates prepared from PRF and UBC membranes similarly activate TGF-β signaling, as indicated by the expression of interleukin 11 (IL-11), NADPH

SUBMITTER: Kargarpour Z 

PROVIDER: S-EPMC9180540 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature