Reference-Guided De Novo Genome Assembly of the Flour Beetle Tribolium freemani.

ABSTRACT: The flour beetle Tribolium freemani is a sibling species of the model organism and important pest Tribolium castaneum. The two species are so closely related that they can produce hybrid progeny, but the genetic basis of their differences has not been revealed. In this work, we sequenced the T. freemani genome by applying PacBio HiFi technology. Using the well-assembled T. castaneum genome as a reference, we assembled 262 Mb of the T. freemani genomic sequence and anchored it in 10 linkage groups corresponding to nine autosomes and sex chromosome X. The assembly showed 99.8% completeness of conserved insect genes, indicating a high-quality reference genome. Comparison with the T. castaneum assembly revealed that the main differences in genomic sequ

SUBMITTER: Volaric M 

PROVIDER: S-EPMC9180572 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature