The Disordered EZH2 Loop: Atomic Level Characterization by ¹H^N- and ¹H^α-Detected NMR Approaches, Interaction with the Long Noncoding HOTAIR RNA.

ABSTRACT: The 96-residue-long loop of EZH2 is proposed to play a role in the interaction with long non-coding RNAs (lncRNAs) and to contribute to EZH2 recruitment to the chromatin. However, molecular details of RNA recognition have not been described so far. Cellular studies have suggested that phosphorylation of the Thr345 residue localized in this loop influences RNA binding; however, no mechanistic explanation has been offered. To address these issues, a systematic NMR study was performed. As the ¹H^N-detected NMR approach presents many challenges under physiological conditions, our earlier developed, as well as improved, ¹H^α-detected experiments were used. As a result of the successful resonance assignment, the obtained chemical shift values indicate th

SUBMITTER: Szabo CL 

PROVIDER: S-EPMC9181245 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature