Dataset Information

Grape ASR Regulates Glucose Transport, Metabolism and Signaling.

ABSTRACT: To decipher the mediator role of the grape Abscisic acid, Stress, Ripening (ASR) protein, VvMSA, in the pathways of glucose signaling through the regulation of its target, the promoter of hexose transporter VvHT1, we overexpressed and repressed VvMSA in embryogenic and non-embryogenic grapevine cells. The embryogenic cells with organized cell proliferation were chosen as an appropriate model for high sensitivity to the glucose signal, due to their very low intracellular glucose content and low glycolysis flux. In contrast, the non-embryogenic cells displaying anarchic cell proliferation, supported by high glycolysis flux and a partial switch to fermentation, appeared particularly sensitive to inhibitors of glucose metabolism. By using different glucose analogs to discriminate between distinct pathways of glucose signal transduction, we revealed VvMSA positioning as a transcriptional regulator of the glucose transporter gene VvHT1 in glycolysis-dependent glucose signaling. The effects of both the overexpression and repression of VvMSA on glucose transport and metabolism via glycolysis were analyzed, and the results demonstrated its role as a mediator in the interplay of glucose metabolism, transport and signaling. The overexpression of VvMSA in the Arabidopsis mutant abi8 provided evidence for its partial functional complementation by improving glucose absorption activity.

SUBMITTER: Parrilla J

PROVIDER: S-EPMC9181829 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Grape ASR Regulates Glucose Transport, Metabolism and Signaling.

Parrilla Jonathan J Medici Anna A Gaillard Cécile C Verbeke Jérémy J Gibon Yves Y Rolin Dominique D Laloi Maryse M Finkelstein Ruth R RR Atanassova Rossitza R

International journal of molecular sciences 20220531 11

To decipher the mediator role of the grape Abscisic acid, Stress, Ripening (ASR) protein, VvMSA, in the pathways of glucose signaling through the regulation of its target, the promoter of hexose transporter VvHT1, we overexpressed and repressed <i>VvMSA</i> in embryogenic and non-embryogenic grapevine cells. The embryogenic cells with organized cell proliferation were chosen as an appropriate model for high sensitivity to the glucose signal, due to their very low intracellular glucose content an ...[more]

PMID: 35682874

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Project description:The biological roles of ASRs (Abscisic acid, Stress, Ripening proteins) involved in certain crucial switches of plant development (grain and pollen germination, senescence, fruit ripening), and responses to environmental cues (drought, salinity, cold, nutrient depletion, fungus infection, heavy metal toxicity) remain still elusive. The functional duality of ASRs, as chaperones for direct protection of biological macromolecules, and as transcriptional regulators of gene expression, needs in-depth understanding of the underlying mechanisms. To further elucidate the biological functions of ASRs a quantitative proteomic analysis (iTRAQ) was performed comparing the nuclear proteins of grapevine embryogenic 41B cells: wild-type (WT) and genetically modified VvMSA-RNAi cells, silenced for grape ASR. Among all identified proteins, thirty-one were differentially expressed in WT and VvMSA-silenced cells and may be classified into five functional groups: metabolism, stress responses, ribosome biogenesis, transcription and chromatin architecture/remodeling. The impact of VvMSA silencing was also confirmed at the level of the genes encoding the differentially expressed nuclear proteins, and the revealed most pronounced effects at protein level suggest a more direct correlation to the putative biological function. Remarkably, many of the thirty-one differentially expressed proteins are supposedly involved in epigenetic events. To further corroborate this hypothesis immunoblot assay of Multiscreen type was performed using twenty-one antibodies to different post-translational modifications (PTMs) of histones H3 and H4 in both cell types, WT and VvMSA-RNAi. The subsequent ImageQuant analysis revealed marked semi-quantitative differences in most PTMs of grape histones H3 and H4, with sharp increase of H3K27ac, H3K36me3, H4K5ac and H4K12ac, as well as a strong decrease of H3K27me3 in VvMSA-RNAi cells. These findings provide new insights on a plausible role of VvMSA as an ASR protein involved in epigenetic mechanisms

Dataset Information

Grape ASR Regulates Glucose Transport, Metabolism and Signaling.

Publications

Grape ASR Regulates Glucose Transport, Metabolism and Signaling.

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