N-Glucosylation in Corynebacterium glutamicum with YdhE from Bacillus lichenformis.

ABSTRACT: Corynebacterium glutamicum is traditionally known as a food-grade microorganism due to its high ability to produce amino acids and its endotoxin-free recombinant protein expression factory. In recent years, studies to improve the activities of useful therapeutics and pharmaceutical compounds have led to the engineering of the therapeutically advantageous C. glutamicum cell factory system. One of the well-studied ways to improve the activities of useful compounds is glucosylation with glycosyltransferases. In this study, we successfully and efficiently glycosylated therapeutic butyl-4-aminobenzoate and other N-linked compounds in C. glutamicum using a promiscuous YdhE, which is a glycosyltransferase from Bacillus lichenformis. For efficient glucosylation,

SUBMITTER: Amoah OJ 

PROVIDER: S-EPMC9182490 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature