The Storage Conditions of High-Fat Diet Are the Key Factors for Diet-Induced Obesity and Liver Damage.

ABSTRACT: The diet-induced obesity (DIO) mouse model has been widely used for obesity studies. The effects of storage conditions on the composition of nutrients in high-fat diets (HFDs) and their impact on metabolic homeostasis have not been systemically investigated. In the current study, we tested the effects of HFDs stored under different conditions and found that mice fed a HFD stored in the fridge (HFD^fri) gained less weight than those fed HFDs stored in the freezer (HFD^fre). Further analysis revealed that changes in the relative abundance of medium-chain triglyceride (MCT) in the HFD^fri, which have much lower intestinal absorption rates, contributed to the body weight differences. In contrast, exacerbated liver damage and elevated levels of unfolded protein res

SUBMITTER: Yi C 

PROVIDER: S-EPMC9182774 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature