Copper monooxygenase reactivity: Do consensus mechanisms accurately reflect experimental observations?

ABSTRACT: An important question is whether consensus mechanisms for copper monooxygenase enzymes such as peptidylglycine monooxygenase (PHM) and dopamine β-monooxygenase (DBM) generated via computational and spectroscopic approaches account for important experimental observations. We examine this question in the light of recent crystallographic and QMMM reports which suggest that alternative mechanisms involving an open to closed conformational cycle may be more representative of a number of experimental findings that remain unaccounted for in the canonical mononuclear mechanisms. These include (i) the almost negligible reactivity of the catalytic copper site (CuM) with oxygen in the absence of substrate, (ii) the carbonyl chemistry and in particular the substrate-induced activation exemplified by t

SUBMITTER: Welch EF 

PROVIDER: S-EPMC9183205 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature