Single-Cell Sequencing of Immune Cell Heterogeneity in IgG4-Related Disease.

ABSTRACT:

Background

The IgG4-related disease (IgG4-RD) is an immune-mediated disorder with fibrotic manifestations. However, the transcriptional profiles of immune cell subsets at single-cell level are unknown. Herein, single-cell sequencing was used to assess the specific cell subpopulations and pathways in peripheral blood mononuclear cells (PBMCs) of IgG4-RD.

Methods

Single-cell sequencing was performed using the PBMCs from four patients with IgG4-RD and three healthy controls (HCs). Functional enrichment and cell analysis were performed through re-clustering of PBMCs to assess functional pathways and intercellular communication networks in IgG4-RD. Western blot and flow cytometry were used to verify sequencing and functional enrichment results.

Results

Four major cell type

SUBMITTER: Wu X 

PROVIDER: S-EPMC9184520 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature