Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein.

ABSTRACT: Membraneless organelles (MLOs) formed via protein phase separation have great implications for both physiological and pathological processes. However, the inability to precisely control the bioactivities of MLOs has hindered our understanding of their roles in biology, not to mention their translational applications. Here, by combining intrinsically disordered domains such as RGG and mussel-foot proteins, we create an in cellulo protein phase separation system, of which various biological activities can be introduced via metal-mediated protein immobilization and further controlled by the water-soluble chlorophyll protein (WSCP)-a remarkably stable, red-light-responsive singlet oxygen generator. The WSCP-laden protein condensates undergo a liquid-to-solid phase transition on light exposure,

SUBMITTER: Li M 

PROVIDER: S-EPMC9184582 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature