Efferocytosis requires periphagosomal Ca²⁺-signaling and TRPM7-mediated electrical activity.

ABSTRACT: Efficient clearance of apoptotic cells by phagocytosis, also known as efferocytosis, is fundamental to developmental biology, organ physiology, and immunology. Macrophages use multiple mechanisms to detect and engulf apoptotic cells, but the signaling pathways that regulate the digestion of the apoptotic cell cargo, such as the dynamic Ca²⁺ signals, are poorly understood. Using an siRNA screen, we identify TRPM7 as a Ca²⁺-conducting ion channel essential for phagosome maturation during efferocytosis. Trpm7-targeted macrophages fail to fully acidify or digest their phagosomal cargo in the absence of TRPM7. Through perforated patch electrophysiology, we demonstrate that TRPM7 mediates a pH-activated cationic current necessary to sustain phagosomal acidification. Using m

SUBMITTER: Schappe MS 

PROVIDER: S-EPMC9184625 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature