Interval-Based Secretomics Unravels Acute-Phase Response in Hepatocyte Model Systems.

ABSTRACT: Mass spectrometry-based secretomics approaches frequently utilize serum-free culture conditions to circumvent serum-induced interference and to increase analytical depth. However, this can negatively affect a wide range of cellular functions and cell viability. These effects become particularly apparent when investigating transcriptionally regulated secretion events and feedback-loops in response to perturbations that require 48 h or more to fully manifest. We present an "interval-based" secretomics workflow, which determines protein secretion rates in short serum-free time windows. Relative quantification using tandem mass tags enables precise monitoring of time-dependent changes. We applied this approach to determine temporal profiles of protein secretion in the hepatocyte model cell lin

SUBMITTER: Knecht S 

PROVIDER: S-EPMC9184749 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature