Involvement of Ca²⁺-ATPase in suppressing the appearance of bovine helically motile spermatozoa with intense force prior to cryopreservation.

ABSTRACT: In cattle, cryopreserved spermatozoa are generally used for artificial insemination (AI). Many of these specimens exhibit helical movement, although the molecular mechanisms underlying this phenomenon remain unclear. This study aimed to characterize helically motile spermatozoa, investigate the involvement of Ca²⁺-ATPase in suppressing the appearance of these spermatozoa prior to cryopreservation, and examine the potential of helical movement as an index of sperm quality. In the cryopreserved semen, approximately 50% of spermatozoa were helically motile, whereas approximately 25% were planarly motile. The helically motile samples swam significantly faster than those with planar movement, in both non-viscous medium and viscous medium containing polyvinylpyrrolidone. In contrast,

SUBMITTER: Duritahala 

PROVIDER: S-EPMC9184823 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature