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Measuring trogocytosis between ovarian tumor and natural killer cells.


ABSTRACT: Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021).

SUBMITTER: Delgado-Gonzalez A 

PROVIDER: S-EPMC9185020 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Measuring trogocytosis between ovarian tumor and natural killer cells.

Delgado-Gonzalez Antonio A   Huang Ying-Wen YW   Porpiglia Ermelinda E   Donoso Kenyi K   Gonzalez Veronica D VD   Fantl Wendy J WJ  

STAR protocols 20220607 2


Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details o  ...[more]

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