Integrated multiomic approach for identification of novel immunotherapeutic targets in AML.

ABSTRACT:

Background

Immunotherapy of acute myeloid leukemia has experienced considerable advances, however novel target antigens continue to be sought after. To this end, unbiased approaches for surface protein detection are limited and integration with other data types, such as gene expression and somatic mutational burden, are poorly utilized. The Cell Surface Capture technology provides an unbiased, discovery-driven approach to map the surface proteins on cells of interest. Yet, direct utilization of primary patient samples has been limited by the considerable number of viable cells needed.

Methods

Here, we optimized the Cell Surface Capture protocol to enable direct interrogation of primary patient samples and applied our optimized protocol to a set of samples from patients with

SUBMITTER: Kohnke T 

PROVIDER: S-EPMC9185890 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature