The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer.

ABSTRACT:

Background

Aberrant methylation of EphA7 has been reported in the process of carcinogenesis but not in cervical cancer. Therefore, an integration study was performed to explore the association between EphA7 hypermethylation and cervical cancer and validate the potential value of EphA7 hypermethylation in the diagnosis of cervical cancer.

Methods

We performed an integration study to identify and validate the association between EphA7 methylation and cervical cancer. First, data on EphA7 methylation and expression in cervical cancer were extracted and analyzed via bioinformatics tools. Subsequently, CRISPR-based methylation perturbation tools (dCas9-Tet1/DNMT3a) were constructed to further demonstrate the association between DNA methylation and EphA7 expression. Ultimately, th

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9185921 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature