The Myotube Analyzer: how to assess myogenic features in muscle stem cells.

ABSTRACT:

Background

The analysis of in vitro cultures of human adult muscle stem cells obtained from biopsies delineates the potential of skeletal muscles and may help to understand altered muscle morphology in patients. In these analyses, the fusion index is a commonly used quantitative metric to assess the myogenic potency of the muscle stem cells. Since the fusion index only partly describes myogenic potency, we developed the Myotube Analyzer tool, which combines the definition of the fusion index with extra features of myonuclei and myotubes obtained from satellite cell cultures.

Results

The software contains image adjustment and mask editing functions for preprocessing and semi-automatic segmentation, while other functions can be used to determine the features of nuclei and myot

SUBMITTER: Noe S 

PROVIDER: S-EPMC9185954 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature