Deep Learning-Assisted Nephrotoxicity Testing with Bioprinted Renal Spheroids.

ABSTRACT: We used arrays of bioprinted renal epithelial cell spheroids for toxicity testing with cisplatin. The concentration-dependent cell death rate was determined using a lactate dehydrogenase assay. Bioprinted spheroids showed enhanced sensitivity to the treatment in comparison to monolayers of the same cell type. The measured dose-response curves revealed an inhibitory concentration of the spheroids of IC ₅₀ = 9 ± 3 μM in contrast to the monolayers with IC ₅₀ = 17 ± 2 μM. Fluorescent labeling of a nephrotoxicity biomarker, kidney injury molecule 1 indicated an accumulation of the molecule in the central lumen of the spheroids. Finally, we tested an approach for an automatic readout of toxicity based on microscopic images with deep learning. Therefore, we cre

SUBMITTER: Trondle K 

PROVIDER: S-EPMC9186384 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature