SsDNA is an allosteric regulator of the C. crescentus SOS-independent DNA damage response transcription activator, DriD.

ABSTRACT: DNA damage repair systems are critical for genomic integrity. However, they must be coordinated with DNA replication and cell division to ensure accurate genomic transmission. In most bacteria, this coordination is mediated by the SOS response through LexA, which triggers a halt in cell division until repair is completed. Recently, an SOS-independent damage response system was revealed in Caulobacter crescentus. This pathway is controlled by the transcription activator, DriD, but how DriD senses and signals DNA damage is unknown. To address this question, we performed biochemical, cellular, and structural studies. We show that DriD binds a specific promoter DNA site via its N-terminal HTH domain to activate transcription of genes, including the cell division inhibitor didA A

SUBMITTER: Gozzi K 

PROVIDER: S-EPMC9186387 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature