Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors.

ABSTRACT: Dopamine supports locomotor control and higher brain functions such as motivation and learning. Consistently, dopaminergic dysfunction is involved in a spectrum of neurological and neuropsychiatric diseases. Detailed data on dopamine dynamics is needed to understand how dopamine signals translate into cellular and behavioral responses, and to uncover pathological disturbances in dopamine-related diseases. Genetically encoded fluorescent dopamine sensors have recently enabled unprecedented monitoring of dopamine dynamics in vivo. However, these sensors' utility for in vitro and ex vivo assays remains unexplored. Here, we present a blueprint for making dopamine sniffer cells for multimodal dopamine detection. We generated sniffer cell lines with inducible expression of seven different dopami

SUBMITTER: Klein Herenbrink C 

PROVIDER: S-EPMC9187629 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature