Investigating the ligand agonism and antagonism at the D_2long receptor by dynamic mass redistribution.

ABSTRACT: The signalling of the D₂ receptor (D₂R), a G protein-coupled receptor (GPCR), is a complex process consisting of various components. For the screening of D₂R ligands, methods quantifying distinct second messengers such as cAMP or the interaction of the receptor with β-arrestin, are commonly employed. In contrast, a label-free biosensor technology like dynamic mass redistribution (DMR), where it is mostly unknown how the individual signalling pathways contribute to the DMR signal, provides a holistic readout of the complex cellular response. In this study, we report the successful application of the DMR technology to CHO-K1 cells stably expressing the human dopamine D_2long receptor. In real-time kinetic experiments, studies of D₂R refer

SUBMITTER: Forster L 

PROVIDER: S-EPMC9187652 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature