A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model.

ABSTRACT: Pig-to-human organ transplantation is a feasible solution to resolve the shortage of organ donors for patients that wait for transplantation. To overcome immunological rejection, which is the main hurdle in pig-to-human xenotransplantation, various engineered transgenic pigs have been developed. Ablation of xeno-reactive antigens, especially the 1,3-Gal epitope (GalT), which causes hyperacute rejection, and insertion of complement regulatory protein genes, such as hCD46, hCD55, and hCD59, and genes to regulate the coagulation pathway or immune cell-mediated rejection may be required for an ideal xenotransplantation model. However, the technique for stable and efficient expression of multi-transgenes has not yet been settled to develop a suitable xenotransplantation model. To develop a stab

SUBMITTER: Ko N 

PROVIDER: S-EPMC9187654 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature