Development and validation of a PCR-free nucleic acid testing method for RNA viruses based on linear molecular beacon probes.

ABSTRACT:

Background

RNA viruses periodically trigger pandemics of severe human diseases, frequently causing enormous economic losses. Here, a nucleic acid extraction-free and amplification-free RNA virus testing probe was proposed for the sensitive and simple detection of classical swine fever virus (CSFV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), based on a double-stranded molecular beacon method. This RNA virus probe contains two base sequences-a recognition strand that binds to the specific domain of CSFV N2 or SARS-CoV-2 N, with a fluorophore (FAM) labeled at the 5' end, and a complementary strand (CSFV-Probe B or SARS-CoV-2-Probe B), combined with a quencher (BHQ2) labeled at the 3' end.

Results

Using linear molecular beacon probe technology, the detectio

SUBMITTER: Du F 

PROVIDER: S-EPMC9187886 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature