Microvolume Analysis of Aflibercept in Aqueous Humor Using Mass Spectrometry.

ABSTRACT:

Purpose

To develop a microvolume analytical method for measurement of the aflibercept concentration in human intraocular fluid and plasma.

Methods

We analyzed trace amounts of aflibercept in human aqueous humor using Fab-selective proteolysis and nano-surface and molecular-orientation limited (nSMOL) proteolysis, coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Patients with age-related macular degeneration or diabetic macular edema were recruited. Just after an injection of 50 µL of aflibercept, regurgitate from needle holes was collected with a micropipette pressed to the side of the injection hole within 10 seconds. The median amount of regurgitate was 4 µL (range, 1-18 µL).

Results

In human plasma, the aflibercept concentration ranged betwee

SUBMITTER: Kimura N 

PROVIDER: S-EPMC9187940 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature