Fbxw11 impairs the repopulation capacity of hematopoietic stem/progenitor cells.

ABSTRACT:

Background

The ubiquitin-proteasome system plays important roles in maintaining the self-renewal and differentiation of stem and progenitor cells through highly ordered degradation of cellular proteins. Fbxw11, an E3 ligase, participates in many important biological processes by targeting a broad range of proteins. However, its roles in hematopoietic stem/progenitor cells (HSPCs) have not been established.

Methods

In this study, the effects of Fbxw11 on HSPCs were studied in vitro and in vivo by an overexpression strategy. Real-time PCR was performed to detect the expression of Fbxw11 in hematopoietic subpopulations. Colony-forming assays were performed to evaluate the in vitro function of Fbxw11 on HSPCs. Hoechst 33342 and Ki67 staining was performed to determine the cell-c

SUBMITTER: Wang L 

PROVIDER: S-EPMC9188144 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature